Error assertion roi failed

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I have an image 640x480 and i need to extract a submatrix with this roi: x="216" y="107" width="423" height="255". I try to this:

    Mat frame = imread(path + fileName);
        vector<KeyPoint> keyPoint = vector<KeyPoint>();
        if (!frame.empty())
        {
            //prendo il punto del frame dell'evento interessato
            int width = itc->width;
            int height = itc->height;
            int x = itc->x;
            int y = itc->y;

            Mat bbox = Mat(frame,Rect(itc->x, itc->y, itc->x + itc->width, itc->y + itc->height));

but i have this exception :

Assertion Failed: 0 <= roi.x && 0 <= roi.width && roi.x + roi.width <= m.cols && 0 <= roi.y && 0 <= roi.height && roi.y + roi.height <= m.rows

where is the error? can you help me?