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Problem with template matching in SUB-IMAGE extracted from ORIGINAL-IMAGE.

One whole day I have tried a lot to get all the related templates (with matchtemplate function) in sub-matrix, which i have already extracted from the original image with the mousecallback function. So my code is below for the Matchingfunction,

void CTemplate_MatchDlg::OnBnTemplatematch()

{ namedWindow("reference",CV_WINDOW_AUTOSIZE); while(true) {

 Mat ref = imread("img.jpg");                    //  Original Image   
 mod_ref = cvCreateMat(ref.rows,ref.cols,CV_32F);// resizing the image to fit in picture box
 resize(ref,mod_ref,Size(),0.5,0.5,CV_INTER_AREA);

   Mat tpl =imread("Template.jmg"); // TEMPLATE IMAGE  
  cvSetMouseCallback("reference",find_mouseHandler,0);

  Mat aim=roiImg1.clone(); // SUB_IMAGE FROM ORIGINALIMAGE

     if(select_flag1 == 1)
    {

        // imshow("ref",aim);

        Mat res(aim.rows-tpl.rows+1, aim.cols-tpl.cols+1,CV_32FC1);
                    matchTemplate(aim, tpl, res, CV_TM_CCOEFF_NORMED);
        threshold(res, res, 0.8, 1., CV_THRESH_TOZERO);

     while (1) 
   {
    double minval, maxval, threshold = 0.8;
    Point minloc, maxloc;
    minMaxLoc(res, &minval, &maxval, &minloc, &maxloc);

   //// Draw Bound boxes for detected templates in sub matrix

    if (maxval >= threshold)
     {
        rectangle(
            aim, 
            maxloc, 
            Point(maxloc.x + tpl.cols, maxloc.y + tpl.rows), 
            CV_RGB(0,255,0), 1,8,0
        );
        floodFill(res, maxloc, cv::Scalar(0), 0, cv::Scalar(.1), cv::Scalar(1.));
          }else
        break;
        }
     }
      ////Bounding box for ROI  selection with mouse
      rectangle(mod_ref, rect2, CV_RGB(255, 0, 0), 1, 8, 0);
    imshow("reference", mod_ref);
    waitKey(10);
    }

//cvReleaseMat(&mod_ref); destroyWindow("reference"); }

// Implement mouse callback

void find_mouseHandler(int event, int x, int y, int flags, void* param) { if (event == CV_EVENT_LBUTTONDOWN && !drag) { /* left button clicked. ROI selection begins*/ point1 = Point(x, y); drag = 1;

}

if (event == CV_EVENT_MOUSEMOVE && drag)
{
    /* mouse dragged. ROI being selected*/ 
    Mat img3 = mod_ref.clone();
    point2 = Point(x, y);
    rectangle(img3, point1, point2, CV_RGB(255, 0, 0), 1, 8, 0);
    imshow("reference", img3);

    //  
}

if (event == CV_EVENT_LBUTTONUP && drag)
{

    Mat img4=mod_ref.clone();
            point2 = Point(x, y);
    rect2 = Rect(point1.x,point1.y,x-point1.x,y-point1.y);
            drag = 0;
    roiImg1 = mod_ref(rect2);  //SUB_IMAGE MATRIX
        imshow("reference", img4);
}

if (event == CV_EVENT_LBUTTONUP)
{
   /* ROI selected */
    select_flag1 = 1;
    drag = 0;
}

}

build and debugging process successfully done. But, when I click the Match button in dialog I m getting the below error,

Unhandled exception at 0x74bf812f in Match.exe: Microsoft C++ exception: cv::Exception at memory location 0x001ae150..

So my idea is to get all the matches in the Sub-image when compare with the TEMPLTE IMAGE and show the final result (matches with bounding boxes) in the ORIGINAL IMAGE itself.

Anyone help me in this regard!! Help would be appreciated greatly!!