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Calculate the location of object on solid background..

If I have an image like this: http://i.imgur.com/3jbQNWg.jpg

What would be the best way to calculate the location of the dot on the image? Currently I'm learning to do this with solid images, but plan to end up doing this with a web cam. Essentially I'm using image subtraction because my background will never change.

Any tips would be appreciated.