I want to know what are the types of image standardizations techniques available. For example, I'm working on hemoglobin concentration detection using photographs in which the patient pull lower conjunctiva downwards with one hand while holding a color calibration card in the other hand. All images are standardized to enable comparison using a previously established method. First, each image was split into its component 8-bit red, green and blue channels. Each channel’s brightness was adjusted by multiplying its brightness by 200/MB where MB is the mean brightness of the color calibration card’s white square. At this point, the channels were duplicated, with one set merged to produce a 24-bit white-balanced image.
This image standardization technique is not perfect and many times give wrong results. Is there any better image standardization technique available. Any idea or right direction will be helpful.